in src/main/scripts/rna_expression_fastq.py [0:0]
def main(script_name, argv):
species, read_type, job_name, dir_out, fastq_list, fastq_list_r2, cufflinks_library_type, library_type, project, \
run, toolset, flag_xenome, cores_per_sample, verbose, sync, genome_load, sample_name_list, master_mode = \
parse_arguments(script_name, argv)
if not library_type:
library_type = "RNASeq"
if not job_name:
job_name = "{}_job".format(library_type)
if not run:
run = "{}_run".format(library_type)
if not genome_load:
genome_load = "LoadAndRemove"
global_config = GlobalConfig(species, read_type, TEMPLATE, WORKFLOW_NAME, toolset)
if species == "human":
global_config_tool_template_name = GLOBAL_CONFIG_TOOL_TEMPLATE_NAME_HUMAN
elif species == "mouse":
global_config_tool_template_name = GLOBAL_CONFIG_TOOL_TEMPLATE_NAME_MOUSE
else:
raise RuntimeError('Failed to determine "species" parameter. Available species: "human"/"mouse"')
global_config_path = global_config.create(global_config_tool_template_name, None, flag_xenome=flag_xenome,
cores_per_sample=cores_per_sample, genome_load=genome_load)
if os.path.isdir(fastq_list):
fastq_list = FastqSampleManifest(read_type).create_by_folder(fastq_list, WORKFLOW_NAME, library_type)
elif 'fastq.gz' in str(fastq_list).split(',')[0]:
sample_name_list = list(str(sample_name_list).split(',')) if sample_name_list else None
fastq_list = FastqSampleManifest(read_type).create_by_list(list(str(fastq_list).split(',')),
list(str(fastq_list_r2).split(',')),
WORKFLOW_NAME, library_type,
sample_names=sample_name_list)
study_config = StudyConfig(job_name, dir_out, fastq_list, cufflinks_library_type, library_type, run,
project=project)
study_config_path = study_config.parse(workflow=WORKFLOW_NAME)
Launcher.launch(global_config_path, study_config_path, sync, java_path=global_config.java_path, verbose=verbose,
master=master_mode)