in src/main/java/com/epam/fonda/tools/impl/Count.java [104:140]
private CountFields constructFields(Configuration configuration, TemplateEngine templateEngine) {
checkValues(configuration);
CountFields countFields = new CountFields();
countFields.jarPath = PipelineUtils.getExecutionPath(configuration);
countFields.cellRanger = configuration.getGlobalConfig().getToolConfig().getCellranger();
countFields.rScript = configuration.getGlobalConfig().getToolConfig().getRScript();
countFields.sampleName = sample.getName();
countFields.transcriptome = configuration.getGlobalConfig().getDatabaseConfig().getTranscriptome();
countFields.libraries = createLibraryCsvFile(configuration, templateEngine);
final String featureRef = configuration.getGlobalConfig().getDatabaseConfig().getFeatureRef();
countFields.featureRef = removeFeatureRefFlag(featureRef) ? NA : featureRef;
countFields.countOutdir = format("%s/%s", configuration.getCommonOutdir().getRootOutdir(), "count");
PipelineUtils.createDir(countFields.countOutdir);
countFields.genomeBuild = configuration.getGlobalConfig().getDatabaseConfig().getGenomeBuild();
countFields.expectedCells = configuration.getGlobalConfig().getCellrangerConfig().getCellrangerExpectedCells();
countFields.forcedCells = configuration.getGlobalConfig().getCellrangerConfig().getCellrangerForcedCells();
countFields.nosecondary = configuration.getGlobalConfig().getCellrangerConfig().getCellrangerNosecondary();
countFields.chemistry = configuration.getGlobalConfig().getCellrangerConfig().getCellrangerChemistry();
countFields.r1Length = configuration.getGlobalConfig().getCellrangerConfig().getCellrangerR1Length();
countFields.r2Length = configuration.getGlobalConfig().getCellrangerConfig().getCellrangerR2Length();
countFields.lanes = configuration.getGlobalConfig().getCellrangerConfig().getCellrangerLanes();
countFields.indices = configuration.getGlobalConfig().getCellrangerConfig().getCellrangerIndices();
String samplePath = format("%s/%s", countFields.countOutdir, countFields.sampleName);
countFields.bam = format("%s/%s/possorted_genome_bam.bam", samplePath, CELLRANGER_OUTPUT_FOLDER);
countFields.matrixInfo = format("%s/%s/filtered_feature_bc_matrix", samplePath,
CELLRANGER_OUTPUT_FOLDER);
countFields.numThreads = configuration.getGlobalConfig().getQueueParameters().getNumThreads();
String countTargetPanel = configuration.getGlobalConfig().getDatabaseConfig().getCellrangerCountTargetPanel();
countFields.targetPanel = StringUtils.isBlank(countTargetPanel) || NA.equals(countTargetPanel)
? null
: countTargetPanel;
String maxMem = configuration.getGlobalConfig().getQueueParameters().getMaxMem();
countFields.maxMemInGb = StringUtils.isBlank(maxMem)
? null
: maxMem.replaceAll("\\D+", StringUtils.EMPTY);
return countFields;
}